Counter-selection facilitated plasmid construction by homologous recombination in Saccharomyces cerevisiae.

Abstract

BioTechniquesVol. 35, No. 4 BenchmarksOpen AccessCounter-selection facilitated plasmid construction by homologous recombination in Saccharomyces cerevisiaePeter Robert Anderson & Yousef Haj-AhmadPeter Robert Anderson*Address correspondence to Peter Robert Anderson, University of Guelph, Department of Molecular Biology and Genetics, Guelph, ON, N1G 2W1, Canada. e-mail: E-mail Address: panderso@uoguelph.caUniversity of Guelph, GuelphSearch for more papers by this author & Yousef Haj-AhmadBrock University, St. Catharines, ON, CanadaSearch for more papers by this authorPublished Online:24 Sep 2018https://doi.org/10.2144/03354bm05AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit FiguresReferencesRelatedDetailsCited ByBuilding biological foundries for next-generation synthetic biology19 May 2015 | Science China Life Sciences, Vol. 58, No. 7RADOM, an Efficient In Vivo Method for Assembling Designed DNA Fragments up to 10 kb Long in Saccharomyces cerevisiae4 June 2014 | ACS Synthetic Biology, Vol. 4, No. 3Combinatorial Assembly of Large Biochemical Pathways into Yeast Chromosomes for Improved Production of Value-added Compounds28 May 2014 | ACS Synthetic Biology, Vol. 4, No. 1Recent advances in DNA assembly technologies23 June 2014 | FEMS Yeast Research, Vol. 35A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences10 May 2013 | Microbial Cell Factories, Vol. 12, No. 1DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways12 December 2008 | Nucleic Acids Research, Vol. 37, No. 2 Vol. 35, No. 4 Follow us on social media for the latest updates Metrics Downloaded 167 times History Published online 24 September 2018 Published in print October 2003 Information© 2018 Author(s)PDF download