Abstract
Congenital or hereditary cataract is a condition where the lens of the eye is not completely transparent resulting in cloudy or blocked vision. Many genes have been found to influence the formation of a cataract. The gene that has been focused on in this paper is the CRYAA gene which codes for the crystallin A protein, a major constituent protein of the lens. Mutations and misfoldings of this protein have been seen to be a major cause of congenital cataract. So, we did an analysis of the gene and the protein using computational tools. As a solution to the condition, we computationally devised a vector that carries the correct CRYAA gene. This vector could be used during the pre-natal stage of pregnancy for children who are believed to inherit this condition.Int. J. Appl. Sci. Biotechnol. Vol 5(2): 216-221
Highlights
Congenital cataract is a hereditary condition where the lens of the eye becomes cloudy, making it difficult to see
It was confirmed that the protein was the A chain of Alpha A crystallin and it was a chaperone
We obtained our final vector displayed in GENtle (Fig. 12), that encodes the gene to code Alpha A crystallin
Summary
Congenital cataract is a hereditary condition where the lens of the eye becomes cloudy, making it difficult to see. In the case of congenital cataract, there is a mutation in one or more of these genes resulting in aggregation of denatured proteins, forming a cataract (Augusteya, 2004). Since the proteins formed once in the lens are permanent and do not move across layers, we thought of correcting the formation of the crystallin proteins from the first step, that is during the prenatal stages. If the condition is present or likely, we thought of introducing a vector in the lens cells that could suppress the malfunctioning gene and produce the functional CRYAA protein.
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