Abstract
BackgroundAcrylamide has been reported to induce hepato- and nephrotoxicity. The present investigation aims to alleviate the dangerous effects at the histopathological and physiological levels of acrylamide by bee venom and its extracted bradykinin-potentiating factor (BPF). Seventy-five adult male mice were divided into 15 groups: a control (G1) 15 animals for 30 (G1.1), 45 (G 1.2), and 60 (G 1.3) days of experimental periods; acrylamide-administered G2.1, G2.2, and G2.3 received acrylamide orally (10 mg/kg b.w) daily for 30, 45, and 60 days. Chips-administered G3.1, G3.2, and G3.3 were fed one third of its daily diet by chips for 30, 45, and 60 days. Bee venom- and BPF-treated G4.1 and G4.2 were i.p. injected with bee venom (1.319 mg/kg b.w.) and BPF (2.314 mg/kg b.w.), respectively, day after the other day for 60 days. G5.1 and G5.2 received acrylamide orally (10 mg/kg b.w) combined either with i.p. by bee venom (1.319 mg/kg b.w.) or BPF (2.314 mg/kg b.w.) respectively day after the other day for 60 days. G6.1 and G6.2 were fed one third of its daily diet by chips for 60 days combined either with i.p. by bee venom (1.319 mg/kg b.w.) or with BPF (2.314 mg/kg b.w.), respectively, day after the other day for 60 days.ResultsThe results showed that acrylamide administration and chips feeding groups suffer from alteration and histopathological changes in liver and kidney tissues that were accompanied with the increase in liver and kidney physiological biomarker (ALT, AST, urea, creatinine, uric acid) and the decrease in the albumin levels. Acrylamide or chips combined with either bee venom or BPF showed improvement in the level of physiological and histopathological studies.ConclusionThe study concluded the protective role of bee venom and its extracted BPF against acrylamide- and chips-induced hepato- and nephrotoxicity.
Highlights
Acrylamide has been reported to induce hepato- and nephrotoxicity
The aim of this study is to evaluate the biological significance of bee venom (BV) and its extracted bradykinin-potentiating factor (BPF) against acrylamide of chemical source or carbohydraterich food overheating-induced complications in the liver and kidney of male mice
Histological, histochemical, and immunohistochemical results of the liver Histological examination of hematoxylin and eosin-stained sections of control group revealed the normal architecture of the liver; the cells are arranged in cords that interspersed with sinusoids (Fig. 1a)
Summary
Acrylamide has been reported to induce hepato- and nephrotoxicity. The present investigation aims to alleviate the dangerous effects at the histopathological and physiological levels of acrylamide by bee venom and its extracted bradykinin-potentiating factor (BPF). 2, and G2.3 received acrylamide orally (10 mg/kg b.w) daily for 30, 45, and 60 days. Chips-administered G3.1, G3.2, and G3.3 were fed one third of its daily diet by chips for 30, 45, and 60 days. G5.1 and G5.2 received acrylamide orally (10 mg/kg b.w) combined either with i.p. by bee venom (1.319 mg/kg b.w.) or BPF (2.314 mg/kg b.w.) respectively day after the other day for 60 days. G6.1 and G6.2 were fed one third of its daily diet by chips for 60 days combined either with i.p. by bee venom (1.319 mg/kg b.w.) or with BPF (2.314 mg/kg b.w.), respectively, day after the other day for 60 days. The World Health Organization (WHO) estimates a daily dietary intake of acrylamide in the range of 0.3–2.0 mg/kg for the general population (WHO, 2005). Acrylamide is mutagenic and carcinogenic in vitro and in vivo studies (Dearfield et al, 1995; Gamboa da Costa et al, 2003)
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