Abstract
Two new coumarins, 6-hydroxy-7-methyl-3-(4′-methoxyphenyl)-coumarin (1) and 6-hydroxy-5-methoxy-7-methyl-3-(4′-methoxyphenyl)-coumarin (2), together with five known coumarins (3–7), were isolated from the leaves of Nicotiana tabacum. Their structures were determined by means of HR-ESI-MS and extensive 1D and 2D NMR spectroscopic studies. Compounds 1–7 were tested for their anti-tobacco mosaic virus (anti-TMV) activities. Compound 2 showed high anti-TMV activity with inhibition rate of 28.6%. This rate is close to that of the positive control. The other compounds also showed potential anti-TMV activity with inhibition rates in the ranges of 13.7–23.2%, respectively.
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