Abstract
Antioxidant capacity of extra virgin olive oil (EVOO) is associated with the overall content of health-promoting compounds, including biophenols. In this work, antioxidant capacity of polar extracts from 42 EVOO and 3 edible seed oil samples was evaluated by constant-current coulometry, using Br2 electrogenerated at a Pt anode as the titrant and bioamperometric detection of the end-point through Br2 excess. The Coulometrically Determined Antioxidant Capacity (CDAC) of EVOO extracts covered the 8–25 mmol electrons (e-) kg−1 range, while it was lower for seeds oils (≤5 mmol e- kg−1). Average CDAC of EVOO with biophenols ≥ 250 mg kg−1 (15 ± 4 mmol e- kg−1) was significantly higher than EVOO with biophenols < 250 mg kg−1 (10 ± 2 mmol e- kg−1). CDAC is a robust, cheap, and rapid test that could be exploited to classify EVOO relying on antioxidant capacity rather than on HPLC-determined content of biophenols.
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