Abstract
Selected springs in the Pardubice Region were repeatedly examined for analytical and microbiological indicators. Microbiological analyses included the determination of intestinal enterococci, coliform bacteria, E. coli, and the number of colonies cultivated at temperature form 22&deg;C to 36&deg;C. The analyses were carried out according to the relevant CSN ISO norms using the membrane filtration technique and also the alternative cultivation method Colilert<sup>&reg;</sup>/Quanti-Tray 2000<sup>&reg;</sup> quantification method using the defined substrate technology. Out of 11 wells and springs examined, only two satisfied the requirements for potable water with all indicators observed.
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