Abstract
The potential of plant regeneration from sunflower cotyledon explants was studied under conditions either to allow direct regeneration (organogenesis) or callus proliferation with high regeneration capacity. Analysis of histological modifications caused by tissue culture conditions showed that the regeneration protocol described here permitted the development of abundant meristematic centers in discrete regions of the basal part of the cotyledon. The protocol presented here proved to be suitable for the regeneration of 10 genotypes, among 20 assayed, including commercial inbred lines and hybrids of common use for production in Argentina. Furthermore, Agrobacterium infection experiments showed that basal halves of cotyledons were transformed, evidencing tumour proliferation.
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