Cotton TCTP1 gene encoding a translationally controlled tumor protein participates in plant response and tolerance to aphids

Abstract

Cotton aphid (Aphis gossypii Glover) is one of the most important economic pests in the world. Long-term unreasonable usage of insecticides has made cotton aphid developing insecticide-resistance, which frequently leads to serious occurrences of cotton aphid in many regions. It is regarded effective and environmentally friendly to control aphids through utilizing plant resistance. In this study, a translationally controlled tumor protein gene, GhTCTP1, was isolated in cotton. It belongs to TCTP subfamily and encodes a protein of 168 amino acids. GhTCTP1 expression was suppressed in cotton plants under cotton aphid attack, but its expression level was up-regulated in the wounded cotton leaves. The choice test and no-choice test demonstrated that overexpression of GhTCTP1 in Arabidopsis enhanced plant resistance to green peach aphid (Myzus persicae). Quantitative RT-PCR analysis revealed that the defense response genes related to salicylic acid signaling pathway were activated in the GhTCTP1 overexpressing transgenic plants. Content of total amino acids was decreased, and phenylalanine ammonialyase activity was altered in leaves of the transgenic Arabidopsis plants, compared with those in wild type. Furthermore, the callose amount in transgenic Arabidopsis leaves was more than that of wild type. These data suggested that GhTCTP1 might be involved in regulation of plant tolerance to aphids, and can be potentially applied in improving aphid-resistance of crops by genetic manipulation.