Abstract
This study is focused on Pseudomonas putida bacteria transport in porous media in the presence of suspended kaolinite clay particles. Experiments were performed with bacteria and kaolinite particles separately to determine their individual transport characteristics in water‐saturated columns packed with glass beads. The results indicated that the mass recovery of bacteria and clay particles decreased as the pore water velocity decreased. Batch experiments were carried out to investigate the attachment of Pseudomonas putida onto kaolinite particles. The attachment process was adequately described by a Langmuir isotherm. Finally, bacteria and kaolinite particles were injected simultaneously into a packed column in order to investigate their cotransport behavior. The experimental data suggested that the presence of clay particles significantly inhibited the transport of bacteria in water‐saturated porous media. The observed reduction of Pseudomonas putida recovery in the column outflow was attributed to bacteria attachment onto kaolinite particles, which were retained onto the solid matrix of the column. A mathematical model was developed to describe the transport of bacteria in the presence of suspended clay particles in one‐dimensional water‐saturated porous media. Model simulations were in good agreement with the experimental results.
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