Abstract
Uptake of Li + induced by the addition of proline to a cell suspension of Escherichia coli was detected using an Li +-selective electrode. This Li + uptake was inhibited by L-azetidine 2-carboxylic acid, a competitive inhibitor of the proline transport system. Thus, direct evidence for Li +-proline cotransport via the proline transport system was obtained. Kinetic parameters of the Li + uptake were determined.
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