Abstract
Galactosyl oligosaccharides are produced from lactose by enzyme preparations from yeasts, molds, and bacteria (1, 2). It has been suggested that these compounds are synthesized via a transgalactosylation mechanism in which galactosyl units of lactose are transferred to accepting or cosubstrate molecules (3-5). That the transferring enzyme of Saccharomyces jragilis acts by the transgalactosylation mechanism has now been verified. In addition, a number of saccharides have been tested as cosubstrates for the enzyme with a view to determining its cosubstrate specificity. The latter experiments have resulted in the preparation of several new oligosaccharides.
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