Cost-effective synthesis of 2D molybdenum disulfide (MoS2) nanocrystals: An exploration of the influence on cellular uptake, cytotoxicity, and bio-imaging.

Dhirendra Sahoo,Jyoti Shakya,Sushreesangita P Behera,Bhaskar Kaviraj,Mahendra Singh Dhaka

doi:10.1371/journal.pone.0260955

Dhirendra Sahoo, Jyoti Shakya + Show 3 more

Open Access

https://doi.org/10.1371/journal.pone.0260955

Copy DOI

Abstract

Ultrasmall MoS2 nanocrystals have unique optoelectronic and catalytic properties that have acquired significant attraction in many areas. We propose here a simple and economical method for synthesizing the luminescent nanocrystals MoS2 using the hydrothermal technique. In addition, the synthesized MoS2 nanocrystals display photoluminescence that is tunable according to size. MoS2 nanocrystals have many advantages, such as stable dispersion, low toxicity and luminescent characteristics, offering their encouraging applicability in biomedical disciplines. In this study, human lung cancer epithelial cells (A549) are used to assess fluorescence imaging of MoS2 nanocrystals. MTT assay, trypan blue assay, flow cytometry and fluorescence imaging results have shown that MoS2 nanocrystals can selectively target and destroy lung cancer cells, especially drug-resistant cells (A549).

Highlights

Few-layered MoS2 nanocrystal, one of the typical two-dimensional (2D) transition metal dichalcogenide materials, show the unique mechanical, optical, electrical, and chemical properties correlated with their ultrasound atomic layer structure and tendering them an appealing alternative to fluorescent dyes, and have attracted particular attention in the scientific uses
The synthesis process is outlined in the experimental segment
The absorption spectra of MoS2 nanocrystal with a strong excitonic peak near the ultraviolet regime at 224 nm confirm the synthesis of a few nanometer particles [31]

Summary

Introduction

Few-layered MoS2 nanocrystal, one of the typical two-dimensional (2D) transition metal dichalcogenide materials, show the unique mechanical, optical, electrical, and chemical properties correlated with their ultrasound atomic layer structure and tendering them an appealing alternative to fluorescent dyes, and have attracted particular attention in the scientific uses. MoS2 nanocrystals can be used in many biomedical applications because of their tunable size and adequate luminescence properties. It has been widely applied in medicine, drug delivery, diagnostics, and outstanding biocompatibility in living organisms [1,2]. Lung cancer is the leading cause of cancer patient fatalities in the United States. As many Americans die from lung cancer each year as prostate, breast and colon cancers combined. Chemotherapy has always been one of the most common ways to treat cancer over the last few decades. Chemotherapy creates some remedial barriers, such as serious side effects, low solubility and a tendency to drug resistance [3,4,5]. The trade-in nanotechnology is growing rapidly, and nanoparticles apply to a variety

Objectives

Results

Conclusion