Abstract
The present study describes the treatment of sugar industry waste water and its use as a potential low cost substrate for production of bioplastic by Bacillus subtilis NG05. The B. subtilis NG05 grow at the rate of 0.14 g h−1 L−1 of production media used and accumulate the 50.1 % of poly β-hydroxybutyrate (PHB). The phase contrast microscopy revealed the presence of PHB granules in B. subtilis NG05 which was further confirmed by Fourier transform infrared spectroscopy and 1H-nuclear magnetic resonance. The polymer was further analysed by differential scanning calorimetry. PHB production yield was achieved up to 4.991 g L−1 with Sugar industry waste water as sole nutrient source. Thus the process provided dual benefits of conversion of a waste material into value added product, PHB and waste management.
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