Abstract

The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera with embedded lens was used instead of photodiodes or an industrial camera. A compact filter wheel using a sliding tape is integrated, and the excitation LEDs are fixed at a 45° angle near the PCR chip, eliminating the need of additional filter wheels. The results show precise positioning of the filter wheel with an error less than 20 μm. Fluorescence detection results using a reference dye and standard DNA amplification showed comparable performance to that of the photodiode system.

Highlights

  • Infectious diseases continue to impose a major burden to global health and the economy [1,2,3,4]

  • Test equipment.that the proposed standard. Chlamydia trachomatis these results demonstrate multiplex qPCR is suitable as POC test equipment

  • The system can be employed to detect four different fluorescence signals using other polymerase chain reaction (PCR) chips that have a similar size with the printed circuit board (PCB)-based PCR chip presented

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Summary

Introduction

Infectious diseases continue to impose a major burden to global health and the economy [1,2,3,4]. In the case of influenza, a sensitive and rapid diagnostic device to detect and analyze the virus is essential in preventing further spread [5]. Research shows that prompt detection and analysis of influenza for children with fever at emergency rooms results in the reduction of time, cost, and need of additional diagnosis [6,7,8,9]. Emerging technologies that increases the efficiency and reduces the time of diagnosis has led to the development of various rapid diagnostic platforms which can be employed at point-of-care (POC) [10,11]. By applying POC devices to diagnose just four common diseases, including bacterial pneumonia, syphilis, malaria, and tuberculosis can prevent

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