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Abstract
In vitro multi-electrode array (MEA) technology is nowadays involved in a wide range of applications beyond neuroscience, such as cardiac electrophysiology and bio-interface studies. However, the cost of commercially available acquisition systems severely limits its adoption outside specialized laboratories with high budget capabilities. Thus, the availability of low-cost methods to acquire signals from MEAs is important to allow research labs worldwide to exploit this technology for an ever-expanding pool of experiments independently from their economic possibilities. Here, we provide a comprehensive toolset to assemble a multifunctional in vitro MEA acquisition system with a total cost 80% lower than standard commercial solutions. We demonstrate the capabilities of this acquisition system by employing it to i) characterize commercial MEA devices by means of electrical impedance measurements ii) record activity from cultures of HL-1 cells extracellularly, and iii) electroporate HL-1 cells through nanostructured MEAs and record intracellular signals.
Highlights
Electrophysiological recording of in vitro cell cultures has been the preferred method for investigating neurons in terms of synaptic activity and ion channel currents [1,2]
Commercial in vitro systems offer higher vertical resolution down to
We provide a complete set of tools for implementing a low-cost in vitro multi-electrode arrays (MEA) acquisition system and for performing recording of spontaneous activity of electrogenic cells, both extracellular and intracellular by means of integrated electroporation capabilities
Summary
Electrophysiological recording of in vitro cell cultures has been the preferred method for investigating neurons in terms of synaptic activity and ion channel currents [1,2]. Multi-electrode arrays (MEA) have been extensively used for characterizing information processing and computation in complex neuronal networks cultured in vitro on the millimeter scale [3]. Electrophysiological recordings of cardiac cells with MEAs are gaining strong interest from the drug discovery and development community[4,5,6], which exploits this technique to characterize drug effects in vitro. The Comprehensive In vitro Proarrhythmia Assay (CiPA) initiative, promoted by the major global drug administration agencies, has indicated the MEA technology as a promising.
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