Abstract
Corynebacterium coyleae is part of the commensal microflora of the skin, urethra, mucous membranes, and genital tract. Isolates from patients with urinary tract infection (UTI) were reported, but the pathogenic potential of this species has not been defined yet. The aim of the study is to determine whether C. coyleae could be the etiological agent of UTI and to analyze its antibiotic susceptibility. Urine samples were cultured quantitatively according to accepted laboratory procedures. The identification of bacterial isolates was carried out using the Vitek MS (bioMérieux) and antibiotic susceptibility was tested using disc diffusion according to EUCAST guidelines. Between 1 January 2017 and 30 October 2018, a total of 39 C. coyleae strains were isolated. This represented 0.32% of all urine samples cultured in the laboratory during the collection period. The strains were isolated from samples obtained from 35 women and 3 men (age median for all—64 years). One female patient presented with C. coyleae in her urine twice at an interval of 21 months. In six cases of UTI, C. coyleae was isolated in monoculture. The isolates had the same resistance pattern. A total of 11 strains were obtained from cases with a clinical diagnosis of UTI. In 13 cases, the strain was cultured in a monoculture and in 28 cases with accompanying species. All strains were susceptible to vancomycin. However, resistance to ciprofloxacin was observed for 58.4% of the strains. Urine isolates of C. coyleae must be considered as contamination or normal flora in most cases (28/39, 72%). In the remaining cases, it can be considered as potential etiologic agents, mostly in women and especially in the 6 UTI cases where C. coyleae was found as the single culture-positive species. Several of these isolates demonstrate resistance to antibiotics commonly used in empiric treatment of urinary tract infections.
Highlights
Corynebacterium coyleae was isolated for the first time in 1997 by Funke et al [1] from the blood cultures of six patients with episodes of fever of unknown origin; one patient was infected with the human immunodeficiency virus (HIV), whereas the other five patients had a previous surgical intervention as the underlying condition
We present the biggest collection of clinical C. coyleae strains in current literature
The results of studies performed by other researchers show that Corynebacterium species, those taxa found as part of the normal microflora, are prominent contaminants of clinical materials, occasionally it is difficult to correctly decide if presence of such bacteria implies contamination or has clinical relevance
Summary
Corynebacterium coyleae was isolated for the first time in 1997 by Funke et al [1] from the blood cultures of six patients with episodes of fever of unknown origin; one patient was infected with the human immunodeficiency virus (HIV), whereas the other five patients had a previous surgical intervention as the underlying condition. Resistance to beta-lactam antibiotics has not commonly been described in C. coyleae isolates. All isolates tested by Fernandez et al [2] were susceptible to β-lactams, gentamicin, rifampin, tetracycline, vancomycin, linezolid, and. Eur J Clin Microbiol Infect Dis (2019) 38:1339–1342 resistant to clindamycin. Isolates from hospitalized patients reported by Barberis et al [5] demonstrated a significantly higher resistance rate to antibiotics than those from outpatients. The aim of the current study is to determine whether C. coyleae could be an etiological agent of urinary tract infections and to analyze its antibiotic susceptibility
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