Abstract
Objectives: Cortisol, called also “stress hormone” participates in organism’s response to stress situations and enters into complex interactions with the hormonal and immune system of a man. The purpose of the study is to follow the cortisol level in saliva as dependent on the anxiety rate in children. Methods: A screening investigation of 170 children was carried out to evaluate anxiety rate with the help of the revised children’s manifest anxiety scale. 71 children (41 with high and 30 with low anxiety rate) were selected to measure the cortisol in the saliva by ELISA method with Saliva Cortisol Enzyme Immunoassay Kit, product of Salimetrics, USA. Results: The results show a dependence between the subjective psychological test for anxiety evaluation in infant age and the cortisol in saliva. Saliva is used as a non-invasive diagnostic indicator for the changes in cortisol levels. Conclusion: Cortisol is an objective oral biomarker that can be used for evaluation of risky oral environment and its connection to the general psychological status of children. Key World: Cortisol, stress hormone, anxiety rate, saliva, ELISA, psychological test
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