Cortisol hormones profiles in dairy cows under repeat breeding

Abstract

Low milk production in Indonesia can cause by physiological stress that leads by nutrient deficiency in dairy cow. It is important to know the stress degree in dairy cow by evaluate the cortisol as stress hormone under repeat breeding condition. This study aims to find out whether any correlations between cortisol hormone detected from the blood and feces of Friesian Holstein Cross breed dairy cow from smallholder livestock groups that have been exposed with long nutrient deficiency. This long and continue stress has an impact on reproductive disorders of repeat breeding. Positive correlation obtained from this study can be used as a reference for the development of non-invasive methods to determine hormone content in livestock. Forty samples of blood from eight females of Friesian Holstein Crossbreed dairy cows with repeated mating disorders were taken every morning (07.00 a.m.), the feces also collected 24h later. Blood serum collected from blood that already preserved about 24h and feces was preserved in freeze dry condition about 7 days. Freeze dried feces then extracted through maceration method with ethanol. The blood serum and feces extraction were analyzed with Cortisol kit hormone using ELISA and analyzed by repeated measurement and correlation. Data showed that Cortisol hormone collection sample from feces did not affect by the number of cows and repeated days but Cortisol from serum samples affected by the cows. Cortisol average from feces sample was 634,160 ng/ml and from serum sample was 411,365 ng/ml. Correlation between Cortisol hormone from feces and serum had positive and low correlation with coefficient correlation 0,242. It can be concluded that evaluate Cortisol profile should identify the cow condition and suggested through blood serum.