Cortisol dynamics during seawater adaptation of Atlantic salmon Salmo salar.

Abstract

We examined plasma cortisol concentration (PCC), cortisol metabolism, and cortisol protein binding (CPB) in chronically cannulated Atlantic salmon (Salmo salar) during seawater (SW) adaptation. PCC rose significantly 2 h after freshwater (FW) to SW changeover but tended to be lower for 6 days thereafter. Plasma chloride concentrations began to stabilize 24-48 h after entry to SW. Cortisol metabolic clearance rate (MCR) was significantly higher 24 h after SW transfer than in FW; CPB and plasma protein concentration were significantly lower, but cortisol production rate was unchanged. The change in plasma chloride after 24 h in SW was negatively correlated with the change in plasma protein (r = -0.915, n = 9, P less than 0.001). The changes in CPB and plasma protein were correlated with the change in MCR (r = -0.643, n = 14, P less than 0.02; r = -0.850, n = 9, P less than 0.005). We hypothesize that these changes in protein concentration reflect an ion-induced expansion of extracellular volume and lead to increased MCR.