Abstract
An attempt was made to determine the levels of corticosteroids in cerebrospinal fluid, in order to study the role of adrenal cortical hormones in the central nervous system in various clinical conditions. The concentration of adrenal cortical hormones in cerebrospinal fluid is so low that the determination of 17 hydroxycorticosteroids in small volumes of cerebrospinal fluid by a method employing the phenylhydrazine reaction is difficult. In this investigation, a simple fluorometric technic was used for the determination of corticosteroids in small quantitities of cerebrospinal fluid. This method offers adequate sensitivity and accuracy for the measurement of normal or elevated levels of corticosteroids in human cerebrospinal fluid.
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