Corticosteroid and thyroid responses of larval and juvenile turbot exposed to the water‐soluble fraction of crude oil

Abstract

Turbot larvae (24–590 degree C days; 2–32 days post‐hatch) and juveniles (1345 degree C days; 98 days post‐hatch), were exposed for 6 h to 25, 33 and 50% water‐soluble fraction (WSF) of crude oil in either static or flow‐through test systems. Larvae showed generalized primary endocrine responses, identified by elevated whole body cortisol content from as early as 2 days post‐hatch. In older larvae and juveniles, the response was related to the WSF concentration. This dose‐response relationship was not apparent in younger and yolk‐sac larvae. Whole body thyroxine content of turbot larvae exposed to the WSF of crude oil was increased, but triiodothyronine content remained stable. Aromatic hydrocarbon concentrations [benzene, toluene, ethylbenzene and xylene (BTEX) and naphthalenes] remained constant during flow‐through tests, but 65% of the initial level of BTEX hydrocarbons and 40% of the naphthalenes were lost during static exposures. Larval mortalities increased with exposure to an increasing concentration of crude oil WSF. Larval activity was significantly reduced even at the lowest WSF concentration.