Corticogenesis and folding process of the neopallium in the South American plains vizcacha, Lagostomus maximus.

Abstract

The plains vizcacha, Lagostomus maximus, is a precocial hystricomorph rodent with a gyrencephalic brain. This work aimed to perform a time-lapse analysis of the embryonic brain cortical development in the plains vizcacha to establish a species-specific temporal window for corticogenesis and the gyrencephaly onset. Additionally, a comparative examination with evolutionarily related rodents was conducted. Embryos from 40embryonic days (ED) until the end of pregnancy ( 154 ED) were evaluated. The neuroanatomical examination determined transverse sulci at 80ED and rostral lateral and caudal intraparietal sulci around 95ED. Histological examination of corticogenesis showed emergence of the subplate at 43ED and expansion of the subventricular zone (SVZ) and its division into inner and outer SVZs around 54ED. The neocortical layers formation followed an inside-to-outside spatiotemporal gradient beginning with the emergence of layers VI and V at 68ED and establishing the final six neocortical layers around 100ED. A progressive increment of gyrencephalization index (GI) from 1.005±0.003 around 70ED, which reflects a smooth cortex, up to 1.07±0.009 at the end of gestation, reflecting a gyrencephalic neuroanatomy, was determined. Contrarily, the minimum cortical thickness (MCT) progressively decreased from 61ED up to the end of gestation. These results show that the decrease in the cortical thickness, which enables the onset of neocortical invaginations, occurs together with the expansion and subdivision of the SVZ. The temporal comparison of corticogenesis in plains vizcacha with that in relative species reflects a prenatal long process compared with other rodents that may give an evolutionary advantage to L. maximus as a precocial species.