Cortical structure in non-dividing and dividing Diophrys japonica spec. nov. (Ciliophora, Euplotida) with notes on morphological variation

Abstract

The morphology and morphogenesis of Diophrys japonica spec. nov., isolated from the Mie Port, Nagasaki, Japan, were investigated from life and following impregnation with protargol. The new species is recognized by the following characters: Body elliptical in outline and slightly greyish to yellowish in color; size in vivo about 80–120×50–70 μm; pellicle flexible, with underlying granules densely arranged in lines; ciliature comprising about 30–46 adoral membranelles, 4–7 frontal, 1–4 ventral and 4–7 transverse cirri, always 1 left marginal and 3 caudal cirri, and 4 dorsal kineties; usually two macronuclear nodules; fragment kinety with 2–5 dikinetids; marine habitat. The main morphogenetic events are: (1) the opisthe's oral primordium develops de novo in a subsurface pouch near the left transverse cirri; (2) the proter retains the parental AZM except for reorganization of some proximal membranelles; (3) cirral anlagen for the frontal, ventral and transverse cirri in both dividers develop separately from the oral primordium or parental cirri, and are derived from the separation of primary primordia that originate de novo; (4) the anlagen for the left marginal cirrus and fragment kinety also form de novo and separately; (5) dorsal kinety anlagen occur within the parental structures at mid-body and posterior end of the cell, of which the right-most one contributes three caudal cirri from its posterior portion. Based on available ontogenetic data, the author proposes that the numbers of left marginal and caudal cirri can be regarded as reliable characters for species identification, while the numbers of frontal, ventral and transverse cirri are not consistent enough for species distinction. A key to the eleven adequately known species of Diophrys is presented.