Cortical processing of chemosensory and hedonic features of taste in active licking mice.

Abstract

In the last two decades, a considerable amount of work has been devoted to investigating the neural processing and dynamics of the primary taste cortex of rats. Surprisingly, much less information is available on cortical taste electrophysiology in awake mice, an animal model that is taking on a more prominent role in taste research. Here we present electrophysiological evidence demonstrating how the gustatory cortex (GC) encodes the basic taste qualities (sweet, salty, sour, and bitter) and water when stimuli are actively sampled through licking, the stereotyped behavior by which mice control the access of fluids in the mouth. Mice were trained to receive each stimulus on a fixed ratio schedule in which they had to lick a dry spout six times to receive a tastant on the seventh lick. Electrophysiological recordings confirmed that GC neurons encode both chemosensory and hedonic aspects of actively sampled tastants. In addition, our data revealed two other main findings: GC neurons rapidly encode information about taste qualities in as little as 120 ms, and nearly half of the recorded neurons exhibit spiking activity entrained to licking at rates up to 8 Hz. Overall, our results highlight how the GC of active licking mice rapidly encodes information about taste qualities as well as ongoing sampling behavior, expanding our knowledge on cortical taste processing.NEW & NOTEWORTHY Relatively little information is available on the neural dynamics of taste processing in the mouse gustatory cortex (GC). In this study we investigate how the GC encodes chemosensory and palatability features of a wide panel of gustatory stimuli when actively sampled through licking. Our results show that GC neurons broadly encode basic taste qualities but also process taste hedonics and licking information in a temporally dynamic manner.