Abstract
BackgroundCortactin is an important regulator involved in invasion and migration of hepatocellular carcinoma (HCC). The aim of this study was to elucidate the forecasting role of cortactin in resectable HCCs.MethodsWe compared the invasiveness and motility among liver epithelial cell line and HCC cell lines by using Transwell assay and wound healing assay. We further investigated the CTTN mRNA expression by real-time PCR. Next, 91 HCC and 20 normal liver tissue samples were detected by IHC and real-time PCR. Finally, we analyzed the clinicopathologic features and survival time of the HCC cases.ResultsWe identified that HepG2, LM3, and SK-Hep-1 had more invasiveness and motility (P <0.05). Compared with liver epithelial cell line, CTTN expression was higher in LM3, HepG2, and MHCC97-L (P <0.01) and lower in SK-Hep-1 (P <0.05). IHC examination showed cortactin expression was closely relative to TNM stage (AJCC/UICC), cancer embolus, and metastasis (P <0.01). Cortactin overexpression indicated a longer survival time of 52 ± 8.62 months and low expression of a shorter survival time of 20 ± 4.95 months (P <0.01). Cortactin examination has more predictive power in patients with Child-Pugh grade A and BCLC stage 0-B.ConclusionsOverexpression of cortactin is closely associated with poor human HCCs prognosis that caused by cancer embolus and metastasis. Cortactin and CTTN should be used for differentiating varieties of survival for patients after HCC resection.
Highlights
Cortactin is an important regulator involved in invasion and migration of hepatocellular carcinoma (HCC)
To confirm whether cortactin and CTTN expression are related to the survival of HCC patients, we followed up this series of cases and compared the survival data of different expression levels
Invasiveness and motility Compared with QSG-7701, a non-cancerous liver epithelial cell line, the HCC cell lines HepG2, LM3, and SK-Hep-1 had more invasiveness (P
Summary
Cortactin is an important regulator involved in invasion and migration of hepatocellular carcinoma (HCC). To investigate whether cortactin and CTTN could become a sensitive and specific biomarker to predicate HCC prognosis, we first selected five cell lines, including one liver epithelial cell line and four HCC cell lines, to detect their ability of invasion and migration. We compared their expression level of CTTN mRNA. To confirm whether cortactin and CTTN expression are related to the survival of HCC patients, we followed up this series of cases and compared the survival data of different expression levels
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