Corrigendum.

Abstract

RespirologyEarly View CorrigendumFree Access CORRIGENDUM This article corrects the following: Effects of chemical composition on the lung cell response to coal particles: Implications for coal workers' pneumoconiosis Yong Song, Katherine Southam, B. Basil Beamish, Graeme R. Zosky, Volume 27Issue 6Respirology pages: 447-454 First Published online: March 20, 2022 First published: 23 May 2023 https://doi.org/10.1111/resp.14521AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL The authors would like to draw the reader's attention to a correction in the following article: Yong Song, Katherine Southam, B Basil Beamish, Graeme R Zosky. Effects of chemical composition on the lung cell response to coal particles: Implications for coal workers' pneumoconiosis. Respirology. 2022 Jun;27(6):447-454. https://onlinelibrary.wiley.com/doi/10.1111/resp.14246 In this article, we reported 1) K2O was positively associated with cytotoxicity; 2) Fe2O3 was negatively associated with cytokine production. In subsequent experiments in our laboratory, we identified an issue with one of the key ELISAs whereby coal dust was non-specifically binding IL-8 that was secreted by the cells, leading to erroneous underestimation of IL-8 release in response to some of our coal dust samples (1). Therefore, we performed an absorption study of IL-8 in a cell-free system (Figure 1) and used this to adjust our published IL-8 data using correction factors (2) (Figure 2). We then used these corrected values to re-run our statistical models (Table 1). FIGURE 1Open in figure viewerPowerPoint Variable absorption of IL-8 by coal dust. The recombinant IL-8 protein was co- incubated with 19 different coal samples and the vehicle control (particle free) in cell medium. FIGURE 2Open in figure viewerPowerPoint Corrected IL-8 response. IL-8 production was corrected by implementing a correction factor for the data in Figure 2 A and B of the original publication. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the control group. Values are mean (SD) with n = 6 per group for A549 and n = 7 per group for THP-1. TABLE 1. Significant associations between coal chemistry and the cell response identified using stepwise multiple regression. Multivariate linear regression was applied to determine whether these associations were maintained after adjustment for particle size. Stepwise regression analysis Linear regression analysis Cell Response R2 Change β ANOVA (F) p β 95% CI p A549 IL-8 SiO2 0.498 0.653 15.258 <0.001 15.87 7.92–23.82 <0.001 Mn3O4 0.129 −0.443 −9927.98 −25,062 – 5206.97 0.183 P2O5 0.125 −0.367 −1440.13 −4271.55 – 391.28 0.297 THP-1 IL-8 Fixed Carbon 0.526 −0.725 18.887 <0.001 −51.19 −75.83 – −26.55 <0.001 The experiments were performed in triplicate. Values are mean (SD). *p < 0.05, **p < 0.001 compared to the control group. These revised data partially changed the outcome of the analysis of the relationship between coal chemistry and the cellular response from our original publication. We found that the IL-8 response in epithelial cells was now positively associated with SiO2, while the IL-8 response in macrophages was negatively associated with the fixed carbon content of the coal dust. It is important to note that this correction does not impact on our original major conclusion that K2O could be a marker of coal mine dust potency in terms of the risk for coal workers' pneumoconiosis (CWP). The authors apologize for these errors and any confusion it may have caused. REFERENCES 1Yen S, Zosky RG, Song Y. Assessing assay absorption artefacts in in vitro cell responses to particles. bioRxiv [Preprint] posted 19 January 2023. https://www.biorxiv.org/content/10.1101/2023.01.16.524328v1.full 2Herseth JI, Totlandsdal AI, Bytingsvik S, Kaur J, Noer M, Bolling AK. The challenge of obtaining correct data for cellular release of inflammatory mediators after in vitro exposure to particulate matter. Toxicol Lett. 2013; 221(2): 110– 7. Early ViewOnline Version of Record before inclusion in an issue FiguresReferencesRelatedInformation