Abstract

Molecular Ecology ResourcesVolume 11, Issue 1 p. 223-224 Free Access Corrigendum This article corrects the following: Trinucleotide microsatellite loci for the zebra mussel Dreissena polymorpha, an invasive species in Europe and North America Kerry A. Naish, Elizabeth G. Boulding, Volume 1Issue 4Molecular Ecology Notes pages: 286-288 First Published online: April 11, 2005 First published: 13 December 2010 https://doi.org/10.1111/j.1755-0998.2010.02935.xCitations: 3AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Molecular Ecology Notes, 1, 286–288. doi: 10.1046/j.1471-8278.2001.00111.x Clarification of the microsatellite loci developed for the zebra mussel KERRY NAISH* and ELIZABETH G. BOULDING† * School of Aquatic and Fishery Sciences, University of Washington, Box 355020, Seattle, WA 98195-5020, USA, †Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada Table 1 of Naish & Boulding (2001) had incorrect primer sequences for the loci Dpol D8, Dpol D9, and Dpol C5. The mistake was created by a misplaced carriage return between the forward and reverse primers. A corrected version of Table 1 is given on the next page. The authors apologize for this error and any inconvenience caused. Table 1. Description of primer sequences used to amplify trinucleotide microsatellite loci in the zebra mussel Dreissena polymorpha. Reaction conditions are described in the text. Numbers of alleles, and observed (HO) and expected heterozygosity (HE) values were derived from a survey of 12 populations (n = 648) from the North American Great Lakes. Alternative primers for Dpol D8 and Dpol D9 have also been published by Astanei et al. (2005). Locus name Primer sequence Core Sequence of cloned DNA Size of cloned allele Size range (bp) Annealing Temperature (°C) Number of cycles Number of alleles HO/HE GenBank Accession no. Dpol A6 5′-TGCCGGTCTAATAATAGAGTTAAC-3′5′-GTGATTGTGTATCTGCTATAAACC-3′ [{AAT}2ATC]4[{ATT}6ATC]2ATT[AAT]2AAC[AAT]3ATC[ATT]2ATCATTAATCAAGAA[TAA]3TATGTA2[ATA]3[ATT]6 301 301–358 58 27 20 0.658/0.893 AF317427 Dpol B6 5′-CGTTGTTCAAGCAATAAGAAAGAC-3′5′-CGTGTGCTCATGTTTCCTCC-3′ [AAT]28 305 256–352 61 25 32 0.611/0.935 AF317426 Dpol B8 5′-TTTGAACATTAAACATTTGTC-3′5′-CACGTCTAGTGCTACAGTTG-3′ [AAT] 20 170 127–244 50 30 35 0.619/0.931 AF317428 Dpol B9 5′-TTGACAATATCCTGTCTAATG-3′5′-GTTCGTCTACAAGTTTATGTG-3′ [AAT]21 240 217–292 57 30 20 0.741/0.909 AF317429 Dpol C5 5′-GCACTGTCAACGTCACACTTTTG-3′5′-CCTTGCTAACAGCTCGGTTGTATC-3′ [AAT]16 213 187–247 63 27 20 0.819/0.891 AF317430 References Astanei I, Gosling E, Wilson J, Powell E (2005) Genetic variability and phylogeography of the invasive zebra mussel, Dreissena polymorpha (Pallas). Molecular Ecology, 14, 1655– 1666. Naish K-A, Boulding EG (2001) Trinucleotide microsatellite loci for the zebra mussel Dreissena polymorpha, an invasive species in Europe and North America. Molecular Ecology Notes, 1, 286– 288. Citing Literature Volume11, Issue1January 2011Pages 223-224 ReferencesRelatedInformation

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