Corrigendum

Abstract

CorrigendumCorrigendumPublished Online:15 Apr 2013https://doi.org/10.1152/ajpcell.zh0-7226.corr.2013Original articleMoreSectionsPDF (1 MB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInEmailWeChat Volume 304, April 2013Volume 73, April 2013Chhunchha B, Fatma N, Kubo E, Rai P, Singh SP, Singh DP. Curcumin abates hypoxia-induced oxidative stress based-ER stress-mediated cell death in mouse hippocampal cells (HT22) by controlling Prdx6 and NF-κB regulation. Am J Physiol Cell Physiol 304: , 2013. First published January 30, 2013; doi:https://doi.org/10.1152/ajpcell.00345.2012 (http://ajpcell.physiology.org/content/304/7/C636.full).—In results, the histogram in Fig. 3D has an error. The correct histogram (Fig. 3D) is shown below.Fig. 3.Curcumin treatment of HT22 cells attenuated apoptosis and released cell cycle arrest during hypoxic stress. A: photomicrograph of HT22 exposed to 1% O2 with or without curcumin treatment. Curcumin (2 μM) or DMSO vehicle-treated cells were placed inside hypoxic chamber to expose to 1% O2. Forty-eight hours later, photomicrographs were taken. Arrows denote rounded white and rounded dead cells (a: control; b: 1% O2; c: Cur + 1% O2). B: photomicrograph of HT22 exposed to CoCl2 with or without curcumin treatment. Curcumin (2 μM) or DMSO vehicle-treated cells were exposed to 100 or 200 μM of CoCl2. Forty-eight hours later, photomicrographs were taken. Arrows denote rounded white dead cells (a: control; b: Cur only; c: 100 μM CoCl2; d: Cur + 100 μM CoCl2; e: 200 μM CoCl2; f: Cur + 200 μM CoCl2). C: curcumin negatively regulated the vulnerability of HT22 cells to CoCl2-evoked apoptotic cell death. Cells untreated (a: vehicle control), treated with different concentration of CoCl2 alone (c: 100 μM; e: 200 μM) or pretreated with curcumin (b: 2 μM curcumin) followed by CoCl2 treatment (d: 100 μM; f: 200 μM). After 48 h of treatment, apoptosis was evaluated using annexin V-FITC/propidium iodide (PI) staining followed by flow cytometry. Top: representative plots showing annexin V-FITC/PI staining of HT22 cells. The proportion of late or apoptotic cells is shown. Bottom: histogram showing a significant increase of apoptotic cells (compare gray vs. black bars). Data represent means ± SD of 3 independent experiments. **P < 0.001. D: curcumin released CoCl2-induced S-phase and G2-M-phase cell cycle arrest in HT22 cells. Cells were cultured in 100-mm plates and treated with curcumin or DMSO (vehicle) for 12 h and then subjected to different concentrations of CoCl2. Cells were harvested and hypotonically lysed in a PI solution to stain the DNA. Nuclei were analyzed for DNA content by flow cytometry. a–f: same as in C. Percentage of cell population at the G1, S, and G2 phases is shown in histogram.Download figureDownload PowerPointThis article has no references to display. Download PDF Previous Back to Top Next FiguresReferencesRelatedInformationRelated ArticlesCurcumin abates hypoxia-induced oxidative stress based-ER stress-mediated cell death in mouse hippocampal cells (HT22) by controlling Prdx6 and NF-κB regulation 01 Apr 2013American Journal of Physiology-Cell Physiology More from this issue > Volume 304Issue 8April 2013Pages C808-C809 Copyright & PermissionsCopyright © 2013 the American Physiological Societyhttps://doi.org/10.1152/ajpcell.zh0-7226.corr.2013History Published online 15 April 2013 Published in print 15 April 2013 Metrics