Abstract

The FEBS JournalVolume 282, Issue 24 p. 4822-4823 CorrigendumFree Access Corrigendum This article corrects the following: Marine antimicrobial peptide tachyplesin as an efficient nanocarrier for macromolecule delivery in plant and mammalian cells Aastha Jain, Bhoopesh K. Yadav, Archana Chugh, Volume 282Issue 4The FEBS Journal pages: 732-745 First Published online: January 8, 2015 First published: 20 October 2015 https://doi.org/10.1111/febs.13529AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat The authors of ‘Marine antimicrobial peptide tachyplesin as an efficient nanocarrier for macromolecule delivery in plant and mammalian cells’ in FEBS J 282 pages 732–745, wish to correct Fig. 2 and its legend, plus the legends for Figs 3, 5 and 8, as follows: Figure 2Open in figure viewerPowerPoint Internalization of Tpl in root tips of T. aestivum (A) control root tip (without addition of peptide), (B) 5 μm MTat, (C) 5 μm dextran sulfate, (D) 5 μm Tat, (E) 5 μm Tpl, (F) 10 μm Tpl and (G) 20 μm Tpl. The images were taken using epi-fluorescence microscope (Olympus, IX51). Figure legends with correction. Fig. 3. (A) Confocal microscopy images to determine the uptake of Tpl in HeLa cells (scale bar = 20 μm): (a) control (without any peptide treatment), (b) 5 μm MTat, (c) 5 μm dextran sulfate, (d) 5 μm Tat, (e) 30 μm Tpl. PMT voltage was adjusted for all samples to maximize image resolution and intensity (B) Quantitative fluorimetric estimation of uptake of Tpl in HeLa cells (RFU, relative fluorescence units) (C) The effect of Tpl on the viability of HeLa cells at varying concentrations is represented by MTT assay. Fig. 5. Increase in nuclear localization of Tpl as assessed by incubation with varying ratios of labelled: non-labelled Tpl in: (A) protoplasts where (a, a’) 0: 20 μm (labelled: non-labelled peptide), (a, b’) 5: 15, (a, c’) 10:10, (a, d’) 15:5, (a, e’) 20: 0 (B) HeLa cells where (a, a’) 0: 30 μm (labelled: non-labelled peptide), (a, b’) 10: 20, (a, c’) 20: 10, (a, d’) 30: 0. Scale bar = 20 μm. PMT voltage was adjusted for all samples to maximize image resolution and intensity. The images in Fig. 3A(e) and Fig. 5B(a, d’) overlap as they represent the same concentration and sample type. Fig. 8. Uptake of Tpl versus mutated Tpl in (A) protoplasts and (B) HeLa cells as determined by confocal microscopy and fluorimetric analysis. (a) Control (without peptide treatment), (b) MTpl-1, (c) MTpl-2, (d) MTpl-3 and (e) Tpl. Scale bar = 20 μm. PMT voltage was adjusted for all samples to maximize image resolution and intensity. Reference 1Jain A, Yadav BK & Chugh A (2015) Marine antimicrobial peptide tachyplesin as an efficient nanocarrier for macromolecule delivery in plant and mammalian cells. FEBS J 282, 732– 745. doi: 10.1111/febs.13178. Volume282, Issue24December 2015Pages 4822-4823 FiguresReferencesRelatedInformation

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