Abstract
Background: Impaired wound healing is a well-documented phenomenon in experimental and clinical diabetes. Emerging evidence favors the involvement of glucocorticoids (GCs) in the pathogenesis of this diabetic complication. Recent data indicated that a heat-shock protein (HSP) with a molecular weight of about 70 kd is expressed in wound healing and it is under the control of the hypothalamic-pituitary-adrenal axis. In view of these findings, the current study was designed to examine the influence of diabetes and the hypercortisolemic state on the expression of HSP 72/73 during wound healing. Methods: Induction of diabetes was achieved by the intravenous injection of streptozotocin at a dose of 55 mg/kg. Subcutaneously implanted polyvinyl alcohol (PVA) sponges were used as a wound healing model. Control and diabetic animals received, respectively, subcutaneous 30-day timed-release pellets of GC (200 mg) and RU 486 (25 mg). Corresponding animals received placebo pellets. Expression of HSP 72/73 within the PVA sponges was assayed with use of Western blotting and immunohistochemical techniques. Results: GCs caused a Cushing-like syndrome with weight loss and adrenal atrophy. A pronounced accumulation of constitutive HSP 72/73 was observed in the cytoplasm of various cell types including fibroblasts, macrophages, and endothelium of nondiabetic controls. The PVA sponge contents of HSP 72/73 were decreased as a function of diabetes. A similar phenomenon was seen in control animals receiving high doses of GCs. Partial normalization of the associated hyperglycemic and hypercortisolemic states of diabetes with insulin (hyperglycemia) and the GC receptor block RU 486 (hypercortisolemia) ameliorated the diabetes-related decrease in PVA sponge contents of HSP 72/73. Conclusions: The current study provides evidence that both diabetes and the hypercortisolemic state are associated with a reduction in PVA sponge content of HSP 72/73. An amelioration of these changes was achieved by the institution of RU 486 therapy. Although our data may point to the possibility that the diabetes-related decrease in HSP 72/73 is mediated at least in part by GCs, a confirmation regarding this premise awaits further investigation. (Surgery 1999;125:594-601.)
Highlights
Dear Editor: On behalf of all the authors, I would like to correct some errors on page 412 of our article published on Journal of Biomedical Research
Journal of Biomedical Research, 2010; 24(6): 411416):"In 1996, the rate of C. difficile infections in the United States hospitals was 31 cases/10 million patients; by 2003, this rate soared to 61 cases/10 million patients[4]
The study by the Association for Professionals in Infection Control and Epidemiology showed that the detection rate of C. difficile was 13.1% and the incidence was 12.4% in hospitalized patients, a significant increase over the previous data[5]" should be corrected as follows with the corrected information underlined "In 1996, the rate of C. difficile infections in the United States hospitals was 31 cases/100,000 patients; by 2003, this rate soared to 61 cases/100,000 patients[4]
Summary
Dear Editor: On behalf of all the authors, I would like to correct some errors on page 412 (lines 7 to 13) of our article published on Journal of Biomedical Research
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