Corrigendum

Abstract

The FEBS JournalVolume 275, Issue s1 p. iii-iii Free Access Corrigendum This article corrects the following: Poster Presentations Volume 275Issue s1The FEBS Journal pages: 99-437 First Published online: June 28, 2008 First published: 13 March 2014 https://doi.org/10.1111/j.1742-4658.2008.06837.xAboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat The abstract by Niewiadomska-Cimicka et al. [1] contains errors on page 128. The abstract should appear as below: PP2A-46 TATA box-driven jhbp core promoter contains a silencing element A. Niewiadomska-Cimicka1, G. Andruszewska1, M. Kochman1, A. Ozyhar1 and M. Schmidt2 1Department of Biochemistry, Wroclaw University of Technology, POLAND, 2Department of Biotechnology and Food Microbiology, Agricultural University of Poznan, POLAND Introduction: Juvenile hormone (JH), regulates insect's development and reproduction. The juvenile hormone binding protein (JHBP) participates in JH signal transmission from corpora allata (JH synthesis) to the target tissues. The regulatory mechanism which controls jhbp expression from any organism has not yet been described. In this report the functional studies of jhbp core promoter from G. mellonella are presented. Methods: The jhbp promoter fragment encompassing −39 /+51 bp from the transcription start point (tsp) and its deletional mutants were cloned into the promoterless pGL3-Basic firefly luciferase reporter vector. Constructs were assayed for transcriptional activity in the Trichoplusia ni High Five cell line and normalized on the basis of Renilla luciferase activities. Results: Our studies revealed the presence of the following putative elements in the jhbp promoter: −29TATAAA−24, +14TCAGTA+19, +38AGGTG+42, corresponding to TATAbox, Inr, DPE, respectively. We have found that the region −39 /+51 is responsible for basic transcriptional activity. Furthermore, the luciferase assay has shown, that promoter with deleted +37/+51 fragment exhibited only slight reduction of transcriptional level, whereas deletion in +28/+51 and +19/+51 region caused a 2.5 and 3.5-fold increase of transcriptional activity, respectively. Further deletion +13/+51 encompassing putative Inr element resulted in promoter activity reduction by about 2/3. Conclusions: Functional analysis of jhbp core promoter has shown that it has composite structure with a suppressive region. Moreover the data has shown, that Inr plays a key role in a regulation of basic transcription level. Acknowledgements: This work was supported by Ministry of Science and Higher Education [2PO4A04430]. Reference 1 Niewiadomska-Cimicka A, Andruszewska G, Kochman M, Ozyhar A & Schmidt M (2008) TATA box-driven jhbp core promoter contains a silencing element. FEBS J 275 (Suppl. 1), 128. Volume275, Issues1Special Issue: Abstracts of the 33rd FEBS Congress and 11th IUBMB ConferenceJune 2008Pages iii-iii ReferencesRelatedInformation