Corrigendum

Abstract

Insect Molecular BiologyVolume 24, Issue 4 p. 502-502 CorrigendumFree Access Corrigendum This article corrects the following: Inducing RNA interference in the arbovirus vector, Culicoides sonorensis M. K. Mills, D. Nayduch, K. Michel, Volume 24Issue 1Insect Molecular Biology pages: 105-114 First Published online: October 7, 2014 First published: 25 May 2015 https://doi.org/10.1111/imb.12178AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat In Mills et al., 2015, Insect Molecular Biology Volume 24 Issue 1, pp. 105–114, the authors discovered that an error was introduced inadvertently in the analysis of the quantitative RT-PCR data using the method established by Pfaffl et al. (2001). The changes had the following consequences for the results of the qRT-PCR analyses: (i) transcript levels of dsGFP treatments are closer to untreated controls (UT); (ii) CsIAP1 transcripts were reduced on average by 33% instead of the initially reported 40%; (iii) variation of both dsGFP and dsCsIAP1 transcript levels increased slightly; (iv) the combination of increased variation and lowered reduction in transcript levels resulted in a loss of the originally reported statistical significance (Student's t-test, P=0.07). Figure 4 and its legend (Page 109) were updated: Figure 4. Relative expression of CsIAP1 transcripts after dsRNA injection (changes italicized): Graph depicts CsIAP1 mean transcript levels at 5 dpi for dsGFP and dsCsIAP1-injected midges relative to untreated controls. QRT-PCR results were analyzed using EF1b as reference gene and untreated midges as calibrator condition. On average, mRNA levels of CsIAP1 were unaffected by dsGFP injection, and reduced by 33% after dsCsIAP1 treatment compared to untreated controls (UT). However, expression levels between dsGFP and dsCsIAP1-treated midges were not statistically significantly different (Student's t-test, P = 0.07). Data are presented as mean ± one S.E.M., from four biological replicates (Figure S2). The last paragraph of the Experimental Procedures (Page 112) should read (changes italicized): Fold change for each treatment (dsGFP or dsCsIAP1) was assessed using a modified ΔΔCt method (Pfaffl, 2001), which takes potentially different primer efficiencies (Figure S10) into account. All qRT-PCRs were performed with 3 technical and four biological replicates. Untreated controls were used as the calibrator in each biological replicate. Expression data between dsGFP and dsCsIAP1 treatments were compared statistically with Student's t-test. Statistical analyses were performed using GraphPad Prism software version 5.01 (GraphPad Software Inc., La Jolla, CA, USA). Sentences 5 and 6 of paragraph 5 (Discussion - Page 110) should read (changes italicized): The 33% transcript reduction of CsIAP1 induced by a comparatively high dose of dsRNA (Miller et al., 2012) suggests that the initial RNAi trigger is not amplified in C. sonorensis, and is likely not taken up by all tissues. Similar results have been obtained from closely related Culicidae (Blandin et al., 2002, Zhu et al., 2003). In addition, we observed considerable variation in overall transcript reduction levels between biological replicates. References Pfaffl, M. W. (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45. CrossrefCASPubMedWeb of Science®Google Scholar Mills, M.K., Nayduch, D. and Michel, K. (2015) Inducing RNA interference in the arbovirus vector, Culicoides sonorensis. Insect Mol Biol 24: 105– 114. Wiley Online LibraryCASPubMedWeb of Science®Google Scholar Volume24, Issue4August 2015Pages 502-502 ReferencesRelatedInformation