CORRIGENDUM.

Abstract

CorrigendumCORRIGENDUMPublished Online:11 Mar 2020https://doi.org/10.1152/ajpGI.zh3-7745-corr.2020Original articleMoreSectionsPDF (674 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInEmail Zhou H, Zhou S, Gao J, Zhang G, Lu Y, Owyang C. Upregulation of bile acid receptor TGR5 and nNOS in gastric myenteric plexus is responsible for delayed gastric emptying after chronic high-fat feeding in rats. Am J Physiol Gastrointest Liver Physiol 308: G863–G873, 2015. First published May 15, 2015; doi:10.1152/ajpgi.00380.2014.The Immunofluorescent labeling section in the materials and methods contains an error. The authors inadvertently omitted details of a goat antibody used for staining TRG5 (goat anti-TGR5 antibody, 1100 SC-48685, Santa Cruz Biotechnology). The first paragraph of the Immunofluorescent labeling section should read as follows:Immunofluorescent labeling. Double or triple immunohistochemical staining was performed against Hu, nNOS, and TGR5, as markers of enteric neurons, nNOS, and bile acid receptor, respectively. Whole mount preparations containing the myenteric plexus were washed three times in PBS (5 min each time). After preincubation in a blocking solution containing 5% normal donkey serum and 0.3% Triton X-100 in PBS, the preparations were incubated overnight with the primary antibodies, at room temperature. The following antiserum and antibodies were used: rabbit anti-nNOS (1:200, SC-648, Santa Cruz Biotechnology), rabbit anti-TGR5 (1:250, ab 72608, Abcam, Cambridge, MA), goat anti-TGR5 antibody (1:100, sc-48685, Santa Cruz Biotechnology) and mouse anti-Hu (1:100, A-21271, Invitrogen). After incubation with the primary antibodies, the preparations were washed three times in PBS (5 min each time) and then incubated for 60 min at room temperature with species-specific fluorophore-conjugated secondary antibodies Cy3, aminomethylcoumarin acetate (Jackson ImmunoResearch Laboratories, West Grove, PA), Alexa Fluor 488 (Molecular Probes, Life Sciences Solutions), and Alexa Fluor 350 (donkey anti-rabbit IgG, Molecular Probes). Tissues were then washed three times in PBS (5 min each time). Whole-mount preparations were mounted on gelatin-coated glass slides and coverslipped with antifading aqueous mounting medium (Gel Mount, Sigma-Aldrich). For control experiments, either the primary or the secondary antibodies were omitted. Tissues were examined and photographed by use of a fluorescence microscope (Olympus BX-51, Tokyo, Japan).Panels C and D of the published version of Figure 2 were incorrectly labeled. The correct Figure 2 and revised legend (with antibody details added) appears below:Figure 2.Activation of neuronal nitric oxide synthase (nNOS)- and TGR5-containing neurons in the gastric myenteric plexus following a 2-wk high-fat diet (HFD). A: nNOS- and TGR5-containing neurons were present in significant quantities in the gastric myenteric plexus. Top, rabbit anti-nNOS antibody (1:200, SC-648, Santa Cruz Biotechnology) with mouse anti-HuC/HuD antibody (1:100, A-21271, Invitrogen); Middle, rabbit anti-TGR5 (1:250, ab 72608, Abcam, Cambridge, MA) with mouse anti-HuC/HuD antibody (1:100, A-21271, Invitrogen). Bottom, rabbit anti-nNOS antibody (1:200, SC-648, Santa Cruz Biotechnology) with goat anti-TGR5 antibody (1:100, sc-48685, Santa Cruz Biotechnology). A and B: colocalization of TGR5 and nNOS in Hu-containing neurons. The primary antibodies used for triple immunostaining in B are mouse anti-HuC/HuD antibody (1:100, A-21271, Invitrogen), goat anti-TGR5 antibody (1:100, sc-48685, Santa Cruz Biotechnology) and rabbit anti-nNOS antibody (1:200, SC-648, Santa Cruz Biotechnology). C: 35% gastric neurons expressed nNOS and 43% expressed TGR5. D: 41% nNOS-containing neurons also expressed TGR5 and 35% of TGR5-containing neurons also expressed nNOS in rats fed a regular chow diet (control, CT). A 2-wk HFD caused increases of 9% in nNOS expression and 18% in TGR5 expression (*P < 0.05, B and C), as well as significant increases in neurons coexpressing nNOS and TGR5 (*P < 0.05, B and D). Each bar represents means ± SE.Download figureDownload PowerPointThis article has no references to display. Previous Back to Top FiguresReferencesRelatedInformationRelated articlesUpregulation of bile acid receptor TGR5 and nNOS in gastric myenteric plexus is responsible for delayed gastric emptying after chronic high-fat feeding in rats 15 May 2015American Journal of Physiology-Gastrointestinal and Liver Physiology More from this issue > Volume 318Issue 3March 2020Pages G610-G611 Copyright & PermissionsCopyright © 2020 the American Physiological Societyhttps://doi.org/10.1152/ajpGI.zh3-7745-corr.2020PubMed32157896History Published online 11 March 2020 Published in print 1 March 2020 Metrics Downloaded 299 times