CORRIGENDUM

Abstract

CORRIGENDACORRIGENDUMPublished Online:01 Jul 2008https://doi.org/10.1152/ajpendo.zh1-5359-corr.2008Original articleMoreSectionsPDF (384 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Volume 294, May 2008 Robinson KA, Buse MG. Mechanisms of high-glucose/insulin-mediated desensitization of acute insulin-stimulated glucose transport and Akt activation Am J Endocrinol Metab 294: E870–E881, 2008; doi:10.1152/ajpendo.00644.2008; http://ajpendo.physiology.org/cgi/content/full/294/5/E870.In this article, Fig. 8, on p. E879, was both produced incorrectly and mislabeled. Panels labeled F and G were supposed to be right panels of D and E, respectively, and the actual panels F and G (both left and right) were missing entirely. The correct full Fig. 8 is presented here below, and the legend, which was correct, is reproduced here for the sake of clarity. Fig. 8.Preincubation conditions modify S6 kinase and S6 ribosomal protein phosphorylation when cells are tested at the end of preincubation, omitting the 2-h deprivation of FBS and of low-dose insulin. Experiments were carried out exactly as described for Fig. 7, except that the 15-min acute stimulation with 100 nM insulin was administered at the end of the preincubation period (I), and the nonstimulated cells (B) were lysed at the same time, i.e., the 2-h deprivation of FBS and of low-dose insulin was omitted. A and B: representative gel where cells preincubated in 5 mM glucose or in 25 mM glucose + 0.6 nM insulin are analyzed after (I) or without (B) acute stimulation with 100 nM insulin. A was blotted with an antibody to phosphorylated S6 kinase and B with an antibody to phosphorylated S6 ribosomal protein. C: blot for β-tubulin. D–G: representative gels and quantitative analyses of cells preincubated in 5 or 25 mM glucose with or without 0.6 nM insulin, as well as 5 mM glucose and 25 mM glucose + 0.6 nM insulin treated with 100 nM rapamycin for 30 min, analyzed in the basal condition (D and F) and after 15 min stimulation with 100 nM insulin (E and G). Left: blots are analyzed after developing with an antibody against phosphorylated S6 kinase (D and E) or against phosphorylated S6 ribosomal protein (F and G). Right: blots stripped and analyzed with antibodies against total S6 kinase (D and E) or against total S6 ribosomal protein (F and G); n = 7 for cells analyzed without acute insulin stimulation and 11 for cells after stimulation with 100 nM insulin. In A and B: cells with and without acute insulin treatment were analyzed on the same gel. D–G: cells with and without acute insulin stimulation analyzed on separate gels. *P < 0.04 in D and F; P < 0.03 in E, and P < 0.005 and **P < 0.02 in G, all compared with control, preincubated in 5 mM glucose.Download figureDownload PowerPointThis article has no references to display. Download PDF Previous Back to Top FiguresReferencesRelatedInformationRelated ArticlesMechanisms of high-glucose/insulin-mediated desensitization of acute insulin-stimulated glucose transport and Akt activation 01 May 2008American Journal of Physiology-Endocrinology and Metabolism More from this issue > Volume 295Issue 1July 2008Pages E223-E223 Copyright & PermissionsCopyright © 2008 by American Physiological Societyhttps://doi.org/10.1152/ajpendo.zh1-5359-corr.2008History Published online 1 July 2008 Published in print 1 July 2008 Metrics