Abstract
[This corrects the article DOI: 10.1155/2019/8060962.].
Highlights
We determined the effect of PKA inhibition on oligomerization of Mfn1 and Mfn2 in ONH astrocytes against oxidative stress combined with cAMP elevation
Our results demonstrated that PKA inhibition significantly increased expression levels of oligomerization of Mfn1 and Mfn2 in ONH astrocytes against oxidative stress combined with cAMP elevation (Figure 7(a))
Since PKA inhibition protects ONH astrocytes by increasing Akt phosphorylation at Ser473 against oxidative stress [7] and Akt regulates Bax phosphorylation at Ser184, inhibiting Bax effects on the mitochondria [40], we further determined the effect of PKA inhibition on Akt and Bax phosphorylation in ONH astrocytes against oxidative stress combined with cAMP elevation
Summary
The lysates were centrifuged at 15000 g for 15 min, and the protein amounts in the supernatants were measured by Bradford methods. Proteins (5-10 μg) were separated by SDS/PAGE and electrotransferred to the PVDF membrane.
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