Corrigendum: Lactoferrin ameliorates prostaglandin E2-mediated inhibition of Na+-glucose cotransport in enterocytes

Abstract

J.R. Talukder, A. Griffin, A. Jaima, B. Boyd, and J. Wright. Department of Biology, LeMoyne-Owen College, 807Walker Avenue, Memphis, TN 38126, USA. Corresponding author: Jamilur R. Talukder (e-mail: Jamil_Talukder@loc.edu). Fig. 8. Kinetics of Na+-dependent glucose cotransport (SGLT1) in rat intestinal epithelial (IEC-6) cells. Data are representative of 3 such experiments. IEC-6 cells were grown in complete Dulbecco’s modified Eagle media on 6-well transwell plates. IEC-6 cells were treated with 50 nmol/L of PGE2 (filled circles), the control group received same volume of vehicle (open circles) at 9 days postconfluence for 24 h. Glucose uptake experiments were performed in the presence or absence of Na+ at 10 days postconfluence. 3-O-methyl-D-[1-3H]glucose ([3H]-OMG) uptake values obtained with extracellular Na+-free buffer were subtracted from the total uptake obtained in the presence of Na+ to calculate Na+dependent glucose uptake. The data were analyzed with a GraphPad Prism 5 software and derived from the Michaelis–Menten equation and are presented in Table 1. Fig. 12. Effect of lactoferrin (Lf) on the kinetics of PGE2-induced inhibition of Na+-dependent glucose uptake in rat intestinal epithelial (IEC-6) cells. The data are representative of 3 such experiments. IEC-6 cells were grown in Dulbecco’s modified Eagle media on transwell plates. The treated (Lf+PGE2) group received Lf (150 nmol/L) at 1 h before PGE2 (50 nmol/L) treatment (filled circle), the control group received the same volume of vehicle (open circle) in 9 days postconfluent for 24 h. Uptake experiments were performed in the presence or absence of Na+ in 10 days postconfluent. The data were analyzed with GraphPad Prism 5 software and derived from the Michaelis–Menten equation and are presented in Table 1. 519