Corrigendum for Oda et al., volume 291, 2006, p. C104–C113

Abstract

CorrigendumCorrigendum for Oda et al., volume 291, 2006, p. C104–C113Published Online:19 Apr 2021https://doi.org/10.1152/ajpcell.00614.2005_COROriginal articleMoreSectionsPDF (336 KB)Download PDFDownload PDFPlus ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInEmail Oda T, Hirota K, Nishi K, Takabuchi S, Oda S, Yamada H, Arai T, Fukuda K, Kita T, Adachi T, Semenza GL, Nohara R. Activation of hypoxia-inducible factor 1 during macrophage differentiation. Am J Physiol Cell Physiol 291: C104–C113, 2006. First published February 15, 2006; doi:10.1152/ajpcell.00614.2005.—In the above article, blots in Figs. 2 and 7 were published incorrectly. Below are the corrected figures and revised Fig. 7 legend. The authors assert that the corrections do not affect the conclusions of the figures or the study.Figure 2.Dose- and time-dependent effect of differentiation-inducing agents on HIF-1 protein expression under 20% O2 conditions. THP-1 cells were exposed to the indicated concentration of PMA for 72 h (A), 100 nM PMA for the indicated time (B), or 1 μM ATRA for the indicated time (C), and harvested for immunoblot assay using anti-HIF-1α (top), HIF-1β (middle), or β-actin (bottom) antibody. D: Jurkat T cells were exposed to 100 nM PMA for the indicated time and harvested for immunoblot assay using anti-HIF-1α (top), HIF-1β (middle), or β-actin (bottom) antibody.Download figureDownload PowerPointFigure 7.MAPK, AKT, the translational regulators p70S6K, S6 ribosomal protein, 4E-BP1, and eIF-4E phosphorylation are induced by PMA treatment. A, B, and D: whole cell lysates were prepared and subjected to immunoblot assays using antibodies specific for phosphorylated (Thr-202/Tyr-204), total p42ERK2/p44ERK1 MAPK, phosphorylated (Ser-473) or total AKT. THP-1 cells were exposed to 100 nM PMA for the indicated time under 20% O2 conditions. Three time points in the original figure were spliced out to prepare 7B to combine bands corresponding to time points 48 h and 72 h next to each other, which is indicated in the figure as well. C: human peripheral blood monocytes (mono) were allowed to differentiate to macrophages (macro). In D, whole cell lysates were prepared and subjected to immunoblot assays using antibodies specific for phosphorylated (Thr-389) p70S6K (PS6K), phosphorylated (Ser-235/Ser-236) S6 ribosomal protein (S6R), phosphorylated (Ser-65) 4E-BP1 (P4E-BP1), and phosphorylated (Ser-209) eIF-4E (PeIF-4E).Download figureDownload PowerPointThis article has no references to display. Previous Back to Top FiguresReferencesRelatedInformationRelated articlesActivation of hypoxia-inducible factor 1 during macrophage differentiation 01 Jul 2006American Journal of Physiology-Cell Physiology More from this issue > Volume 320Issue 4April 2021Pages C666-C667 Crossmark Copyright & PermissionsCopyright © 2021 the American Physiological Society.https://doi.org/10.1152/ajpcell.00614.2005_CORPubMed33871285History Published online 19 April 2021 Published in print 1 April 2021 PDF download Metrics Downloaded 256 times