Abstract
[This corrects the article on p. 44 in vol. 4, PMID: 23565118.].
Highlights
Specialty section: This article was submitted to Plant Genetics and Genomics, a section of the journal Frontiers in Genetics
We should note that the primers used for quantitative RT-PCR of the NbROS1 gene listed in Table A1 turned out to have a sequence mismatch with the target
We found that a set of primers without sequence mismatch (5′CCAAGAAGCTGGTAGGCTAT-3′; 5′-GCAAACACCTCGTTAACTT-3′) yielded amplification products at a higher level, both primer sets amplified a single DNA fragment and were valid for quantification, i.e., comparison of the relative level of NbROS1 mRNA between samples
Summary
Specialty section: This article was submitted to Plant Genetics and Genomics, a section of the journal Frontiers in Genetics. Corrigendum: Enhancement of RNA-directed DNA methylation of a transgene by simultaneously downregulating a ROS1 ortholog using a virus vector in Nicotiana benthamiana Research Faculty of Agriculture, Hokkaido University, Sapporo, Japan
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