Abstract
Allen video-enhanced contrast, differential interference-contrast microscopy (AVEC-DIC) combines high resolution differential interference contrast microscopy of the Zeiss IM35 microscope with a Hamamatsu video camera to obtain images with increased magnification, resolution, contrast and visibility. Through this combination and by capitalizing on light diffraction phenomena, it is possible to visualize subcellular organelles, particles, and structures which are an order of magnitude smaller (0.01 μm) than the normal limits of resolution with light microscopy (0.2 μm). The use of gold “finder” grids mounted on glass coverslips provides the mechanism by which cells after being observed in the video system can be located in the electron microscope. Such a correlative approach allows static ultrastructural features to be associated with cellular dynamics as observed and recorded by video. When the electron microscopy is done using either IVEM, HVEM or conventional TEM at 120 keV and the images recorded as stereo pair micrographs, the AVEC-DIC spatial data can be directly compared to high resolution 3-D data obtained by EM.
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