Abstract
Correlative light and electron microscopy is a valuable tool to image samples across resolution scales and link data on structure and function. While studies using this technique have been available since the 1960s, recent developments have enabled applying these workflows to large volumes of cells and tissues. Much of the development in this area has been facilitated through the collaborative efforts of microscopists and commercial companies to bring the methods, hardware and image processing technologies needed into laboratories and core imaging facilities. This is a prime example of how what was once a niche area can be brought into the mainstream of microscopy by the efforts of imaging pioneers who push the boundaries of possibility.
Highlights
Correlative light and electron microscopy is a valuable tool to image samples across resolution scales and link data on structure and function
Since electron microscopists generally used light microscopy (LM) mostly to examine semi-thin sections before taking ultra-thin ones to examine in the transmission electron microscope (TEM), this must have seemed to many an extravagant purchase
Like all complicated techniques correlative light and electron microscopy (CLEM) developed over time with many complex variations, some of which combined multiple advanced techniques such as colloidal gold labelling, live cell video microscopy, scanning electron microscopy (SEM) and high-voltage transmission electron microscopy
Summary
Correlative light and electron microscopy is a valuable tool to image samples across resolution scales and link data on structure and function.
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