Correlations between serum microRNA-210, -204-5p, -376c and hemorheology indexes in patients with pregnancy-induced hypertension and their diagnostic values

Abstract

Objective To investigate correlations between serum microRNA (miRNA)-210, -204-5p, -376c and hemorheology indexes in patients with pregnancy-induced hypertension and their diagnostic values. Methods Eighty patients with pregnancy-induced hypertension admitted in Huanghua Municipal People′s Hospital of Hebei Province from October 2016 to October 2018 were randomly selected as research subjects by simple random sampling method and enrolled into study group (n=80). And 80 normal pregnant women in the second trimester were randomly selected by the same method during the same period in the same hospital as control group (n=80). Real-time quantitative PCR was used to detect relative expression levels of serum miRNA-210, -204-5p, -376 of two groups. Hemorheology indexes were measured in two groups of subjects by automatic blood cell analyzer. Independent-samples t test was used to compare the relative expression levels of serum miRNA-210, -204-5p, -376 and hemorheology indexes between two groups. Area under receiver operating characteristic curve (ROC-AUC) was used to analyze the diagnostic values of relative expression levels of serum miRNA-210, -204-5p, -376 in diagnosis of pregnancy-induced hypertension. Pearson correlation coefficient was used to analyze the correlation between expression levels of serum miRNA-210, -204-5p and -376c and hemorheology indexes in patients with pregnancy-induced hypertension. This study was approved by the Ethics Committee of Huanghua Municipal People′s Hospital of Hebei Province (Approval No. 18101523). Clinical research informed consent was obtained from each participate. There were no statistical differences in the general clinical data such as age, gestational age at the time of enrollment, body mass index before pregnancy, gravidity, parity of patients, and so on (P>0.05). Results ①The relative expression levels of serum miRNA-210 and miRNA-204-5p in study group were (1.56±0.35) and (1.78±0.40), respectively, which were higher than those (0.59±0.11), (0.72±0.15) in control group, respectively, and relative expression level of serum miRNA-376c in study group was (0.33±0.09), which was lower than that (1.24±0.32) in control group, and all the differences between two groups were statistically different (t=23.648, 22.193, 24.485; all P<0.001). ②The ROC-AUC of relative expression levels of serum miRNA-210, -204-5p, and -376c in diagnosis of pregnancy-induced hypertension were 0.824 (95%CI: 0.738-0.902, P<0.001), 0.871 (95%CI: 0.810-0.943, P<0.001), 0.833 (95%CI: 0.746-0.908, P<0.001), respectively. According to the maximum principle of Youden index, the optimal cut-off values of relative expression levels of serum miRNA-210, -204-5p, and -376c in diagnosis of pregnancy-induced hypertension were 0.696, 1.512, and 0.712, respectively. And the sensitivities for diagnosis of pregnancy-induced hypertension were 83.3%, 93.1% and 85.0%, respectively, and the specificities were 85.0%, 75.0% and 85.0%, respectively. ③The wholeblood reduced viscosity (WRV), low shear reduction viscosity (LSRV) and plasma viscosity (PV) of patients with pregnancy-induced hypertension in study group were (7.9±1.5) mPa·s, (17.6±3.6) mPa·s, (1.6±0.3) mPa·s, respectively, which were higher than those (7.2±1.3) mPa·s, (15.6±3.4) mPa·s, (1.2±0.2) mPa·s of healthy pregnant women in control group, respectively, and all the differences were statistically significant (t=3.154, P=0.002; t=3.613, P<0.001; t=9.923, P<0.001). ④There was a positive correlation between relative expression levels of serum miRNA-210 and WRV, LSRV and PV, respectively (r=0.343, P=0.002; r=0.415, P<0.001; r=0.287, P=0.001). There was a positive correlation between relative expression levels of serum miRNA-204-5p and PV (r=0.326, P=0.003). There was a positive correlation between relative expression levels of serum miRNA-376c and WRV, LSRV, respectively (r=0.317, P=0.004; r=0.351, P=0.001). Conclusions Relative expression levels of serum miRNA-210, miRNA-204-5p and miRNA-376c are associated with hemorheology indexes, and can be used as potential biomarkers for the diagnosis of pregnancy-induced hypertension, perhaps. Key words: Hypertension, pregnancy-induced; MicroRNAs; MicroRNA-210; MicroRNA-204-5p; MicroRNA-376c; Hemorheology; Pregnant women