Abstract
The effects of activated charcoal and organic substances on embryogenesis in anther cultures of Anemone canadensis L. were studied. Embryogenesis was independent of the presence in the culture medium of glycine, nicotinic acid, pyridoxine, thiamine, folic acid, d‐biotin or myoinositol. Absence of Fe‐EDTA totally inhibited embryogenesis. Activated charcoal (AC) adsorbed Fe‐EDTA, pyridoxine, folic acid and nicotinic acid in a double‐layer medium almost completely within 24 h. If petri dishes according to the souble‐layer method were stored overnight or more, embryogenesis was totally inhibited, probably due to adsorbtion of Fe‐EDTA by AC. It was shown that AC itself released some yet unidentified substance(s) that stimulated embryogenesis. The addition of polyvinylpolypyrrolidone (PVPP) to the culture medium stimulated embryogenesis, but PVPP was not as efficient as AC. Embryogenesis was totally inhibited when AC and PVPP were applied together. Minor additions of ethanol to the culture medium stimulated embryogenesis when AC was present, but no such effect was obtained when AC was absent.
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