Correlations among climbing fiber responses of nearby cerebellar Purkinje cells in the immature rat.

Abstract

Correlations between pairs of spontaneous climbing fiber responses (CFRs) recorded from couples of nearby Purkinje cells (PCs) were studied in immature rats by using cross-correlograms between CFR pairs, and compaired to those in adult animals. Correlations were found as early as day 3. Some days later, on PN days 7--9, these correlations were higher than in the adult. In most cases, this was apparently not due to the multiple innervation of PC by climbing fibers (CFs) which normally occurs during this immature stage since: 1) temporal relationships between the paired CFRs varied by more than 30 ms and 2) thresholds for pairs of graded CFRs and additional components of the responses evoked in the 2 PCs by juxtafastigial or olivary stimulation were different. Synchronizing mechanisms were therefore likely to be already located at the olivary level. However, in 3 couples of multiply innervated PCs whose spontaneous CF activities were highly correlated, stimulation experiments revealed a common innervation of the 2 cells by branches of the same CF. In multiply innervated cells, spontaneous responses mediated through distinct CFs were also synchronized, suggesting that these fibers originate from neighboring neurons of the inferior olive. Finally, in 7 to 9-day-old rats, correlations among CFR pairs were much more restricted in the longitudinal axis of the folium than in the transverse one. On the whole, the present study indicates that correlations among CFRs of nearby PCs exist as soon as CF-PC synapses are established and the latter are already organized in sagittal strips at early stages of development.