Abstract
BackgroundBladder cancer, including urothelial carcinoma (UC), is the most common malignancy of the urinary tract and the fourth most frequent cancer overall in men. Wnt5a, a member of the Wnt family of proteins, has been shown to have contradictory roles in the pathogenesis of many cancers, acting either as tumor suppressor or tumor promoter. The objective of this study was to investigate the expression and role of Wnt5a in the pathogenesis of UC and suggest possible clinical applications for diagnosis, prognosis and treatment.MethodsWe characterized the expression of Wnt5a in 33 human UC samples using immunohistochemistry. The samples were obtained via transurethral resection, immediately fixed in formalin and then embedded in paraffin. The correlation between Wnt5a immunoreactivity, histological grade, and pathological stage of the tumor was analyzed. The expression of Wnt5a mRNA as well as the effect of Wnt5a on cell migration was also evaluated in two UC cell lines, T24 and J82, and a normal urothelial cell line.ResultsOur immunohistochemical results revealed that Wnt5a staining intensity correlated positively with the histological grade and pathological stage of the UC. Wnt5a mRNA expression differed widely in the three urothelial cell lines, with high levels in one carcinoma cell line and low levels in the other cell line in comparison to the normal urothelial cell line. Migration increased in both UC cell lines in response to Wnt5a treatment.ConclusionsOur results show that the Wnt5a pathway may play a role in the pathogenesis of UC and suggest that Wnt5a may serve as an additional, complementary diagnostic/prognostic marker for UC.Virtual slidehttp://www.diagnosticpathology.diagnomx.eu/vs/1952312091979566
Highlights
Bladder cancer, including urothelial carcinoma (UC), is the most common malignancy of the urinary tract and the fourth most frequent cancer overall in men
We examined the expression of Wnt5a by immunohistochemistry (IHC) in 33 formalin-fixed, paraffin-embedded (FFPE) human UC samples
We found that Wnt5a may be involved in the migration of malignant UC cells, which could have implications regarding the invasiveness of the tumor
Summary
Bladder cancer, including urothelial carcinoma (UC), is the most common malignancy of the urinary tract and the fourth most frequent cancer overall in men. Most of the tumors at pT1 pathological stage, which include tumors with invasion into the lamina propria, are high grade; 30-50% of the tumors in this group will progress to a higher stage [1,2]. These features make UC challenging in clinical practice in relation to diagnosis, prognosis, treatment, or follow-up for each individual patient
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