Correlation of umbilical cord blood volume with CD34+ cells concentration

Abstract

Aims: Umbilical cord blood (UCB) has been recently considered as an alternative source of hematopoitic progenitor cells for clinical application. The parameters commonly used to evaluate a UCB unit and predict transplant outcomes have been total nucleated cell count (TNCs), CD34+ cells concentration and total volume of cord blood collected. The volume of cord blood collection is also important for the high yield of CD34+ cells concentration and TNCs. The aim of the study was to find the correlation of umbilical cord blood volume with cord blood derived CD34+ cells concentration. Methods: Umbilical cord blood was collected from normal vaginal and cesarean deliveries. Total volume of cord blood collection was noted. It was immediately processed and assessed for total nucleated cells count and CD34+ cells concentration. Assessment of maternal and neonatal parameters such as mode of delivery, baby's birth weight and sex, cord blood volume and CD34+ cells concentration was made. Results: Total volume of cord blood and CD34+ cells concentration positively correlated with cesarean delivery and higher birth weight of the baby (p<0.01). We also found that, CD34+ cells concentration was higher in greater volume of collected cord blood. Conclusions: Our study concludes that higher volume of cord blood should be preferred for processing and stem cell infusion.