Correlation of TSP1 G1678A and A2210G Polymorphisms to Risk of Gastric Cardia Adenocarcinoma

Abstract

Objective To investigate the possible association of TSP1 exon10 G1678A and exon13 A2210G polymorphisms with susceptibility to gastric cardia adenocarcinoma(GCA) in a population of North China.Methods The TSP1 G1678A and A2210G single nucleotide polymorphisms(SNPs) were genotyped by polymerase-chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis in 145 GCA patients and 198 healthy controls.Results The overall genotype and allelotype distributions of TSP1 G1678A in GCA patients were not significantly different from those in healthy controls(P0.05).When stratified for smoking status and upper gastrointestinal cancers(UGIC) family history,compared with GG and GA genotype,AA genotype significantly increased the risk of developing GCA in non-smoker group [age and gender and UGIC family history adjusted odds ratio(OR)= 1.79,95%CI= 1.46~2.11].The frequency of G allelotype was less than 1%,which indicated that TSP1 A2210G was not a common polymorphism in north China.Conclusion AA genotype of TSP1 G1678A may be one of the factors that affect the risk of developing GCA in non-smoking population in north China.