Abstract
The correlation between mating behaviour of bulls and patterns of gonadotrophins (GTH, i.e. FSH and LH) and oestradiol-17β (E 2) was examined in oestrous cows, using two bulls. Four cows were placed with each bull in a free stall (10 m × 15 m) at 44 h after the second injection of PGF 2α analogue (cloprostenol). Mating (mounting and ejaculation) behaviour of the bull was observed and serum levels of FSH, LH and E 2 were measured in cows during the next 33 h. The bull ejaculated only with cows in which elevated E 2 levels and a GTH surge occurred. When the time of the GTH peak was designated as 0, and the patterns of hormones in cows with mating behaviour were combined, it was found that 92.3% of ejaculating and 79.1% of mounting behaviour occurred within the range of the GTH surge, indicating that the former behaviour was coincident with the GTH surge.
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