Correlation of testicular artery Doppler velocimetry with kinetics and morphologic characteristics of epididymal sperm in dogs

Abstract

Sperm quality can be affected by a reduction in testicular blood flow, which can be measured by Doppler ultrasonography. The aim of this study was to correlate the Doppler velocimetry of the testicular artery with kinetics of the epididymal spermatozoa in dogs. Twenty-two dogs (44 testicles) were evaluated by Doppler ultrasonography in five regions of the testicular artery before orchiectomy. Spermatozoa were recovered by the epididymal tail compression technique and analysed for kinetics on a computer-assisted semen analysis (CASA system). Morphology (modified Karras) and sperm membrane integrity were analysed by eosin-nigrosine staining. Data were analysed by Pearson's correlation test (p<.01). The mean total motility was 69.0%±17.7, progressive motility was 43.7%±14.7, average path velocity (VAP) was 127.0µm/s±20.7, curvilinear velocity (VCL) was 221.0µm/s±31.1, and sperm velocity index (SVI) was 389.9±56.1. There were positive correlations between the peak systolic velocity (PSV) in the proximal supratesticular region with the SVI (r=.529), VCL (r=.555) and VAP (r=.473), and a negative correlation with the percentage of slow spermatozoa (r=-.463). The results suggest that the testicular artery blood flow velocity can positively affect the speed of spermatozoa movement. For the first time, we have correlated sperm kinetics with the Doppler evaluation of the testicular artery in dogs.