Abstract
Absorption spectra of tomato fruit purees supplemented spectroscopic studies of carotene fractions in hexane solution to trace development of polyenes in ripening fruits, from very green to fully ripe. Genetic types of great diversity ranged from yellow through orange and red in ripe fruit color. Purees indicated early appearance of proneurosporene in all types, while extract data indicated first the appearance of α- or β-carotene isomers. Phytoene, phytofluene, and ζ-carotene varied with different strains. cis-Isomers appear before all- trans isomers in some cases. 3,4,3′,4′-Dehydrolycopene was apparent in all red and some orange fruit purees. Considerable support is given to the theory of ring closure before lycopene formation in polyene synthesis, but paths may differ greatly among genetic types. The spectroscopic study of purees appears a promising tool in studies of polyene analysis.
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