Correlation of SOD and MDA Expression in the Organ of Corti and Changes in the Function of Outer Hair Cells Measured by DPOAE Examination in Noise-Exposed Rat Cochlea.

Siti Hajar Haryuna,Reastuty Reastuty

doi:10.52547/rbmb.10.1.41

Abstract

Hearing loss due to noise can cause the disturbances toward the quality of life and cause mechanical damage and metabolic decompensation. Distortion Product Otoacoustic Emission (DPOAE) is an examination to assess the sensory function of outer hair cells. Superoxide Dismutase (SOD) and Malondialdehyde (MDA) are markers of oxidative stress. The aim of this study was to identify the correlation between DPOAE examination and SOD and MDA expression in rats exposed to noise. This research was conducted on 27 rats which were divided into 3 groups, group 1 (control), group 2, and group 3 were groups with 100 dB and 110 dB noise exposure respectively. Our findings show a decrease in SOD expression and DPOAE values as well as an increase in MDA expression in rats exposed to noise and there is a positive correlation between Signal to Noise Ratio (SNR) value with SOD expression (r= 0.733, p= 0.025) and a negative correlation between SNR value with MDA expression (r= -0.678, p= 0.045). our study find the correlation of oxidant and antioxidant status values in the organ of corti and changes in the function of outer hair cells in noise-exposed rat models.

Highlights

Noise can cause hearing loss both from work and the environment [1,2,3]
Distortion products Otoaccoustic Emissions (DPOAEs) are the result of an active intermodulation process in the cochlea mediated by outer hair cells and obtained simultaneously which indicates the stimulation of two pure tones [12]
In a study conducted by Millon et al in 2018, found that female mice were significantly more protected against Noise Induced Hearing Loss (NIHL) compared to male mice [21]

Summary

Introduction

Noise can cause hearing loss both from work and the environment (not from the work) [1,2,3]. The resulting emission is a response from stimuli originating from various frequencies, so this can be an indicator of sensory function of outer hair cells in the cochlea [11]. Distortion products Otoaccoustic Emissions (DPOAEs) are the result of an active intermodulation process in the cochlea mediated by outer hair cells and obtained simultaneously which indicates the stimulation of two pure tones [12]. Distortion Product Otoacoustic Emission (DPOAE) is an examination to assess the sensory function of outer hair cells. The aim of this study was to identify the correlation between DPOAE examination and SOD and MDA expression in rats exposed to noise. Conclusions: our study find the correlation of oxidant and antioxidant status values in the organ of corti and changes in the function of outer hair cells in noise-exposed rat models

Objectives

Results

Conclusion